Glycan characterization of therapeutic proteins is of utmost importance due to the role of carbohydrates in protein stability, half-life, efficacy and mechanism of action. This application note presents the on-line CE-LIF-MS technology for the direct identification of N-linked glycan profiling of rMAbs. CE-MS technology with on-line LIF detection has been developed to provide accurate mass information on major and minor peaks observed in the routine CE-LIF assay.
9-Aminopyrene-1,4,6-trisulfonate (APTS) is dye that is frequently used for the analysis of mono or oligosaccharides. The labeling of sugars involves a reductive amination of the reductive function of the mono or oligosaccharides followed by reaction with the dye. APTS is routinely used in Capillary Electrophoresis separation. In this note, we analyze oligosaccharides labeled with APTS with a 480nm LED.
Therapeutic Antibodies is a category of therapeutics that plays an important role in controlling many diseases such as cancer, allergy, inflammation, infectious or autoimmune diseases. The characterization of carbohydrates structures on therapeutic proteins is of utmost importance due to their role in clearance and mechanism action. The main techniques for carbohydrates analysis involve high-performance liquid chromatography (HPLC), mass spectrometry (MS) and capillary electrophoresis (CE). 2-aminobenzoic acid (2AB) is a dye frequently used for the analysis of mono or oligosaccharides with HPLC.
This application note describes an alternative method of the selection of aptamers using CE-UV-LEDIF detection/non-SELEX.
In this application note, we optimised the collection parameters of aptamers on a standard mixture to validate the collection method in a small range of migration times to get the slower koff.
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