ZETALIF DETECTORS

ULTRA SENSITIVE FLUORESCENCE DETECTION

The Zetalif Laser or LED induced fluorescence detectors are ultra sensitive solutions for all separation techniques such as Liquid Chromatography (capillary LC, microLC, nanoLC, UPLC) and Capillary Electrophoresis. A large range of LED and Lasers wavelengths is available: 275, 365, 450, 480, 530 and 640 nm.

ZETALIF LED

Light Emitting Diodes (LEDs) are monochromatic light sources exhibiting very long life time (>20000 hours) compared to lasers. This makes Zetalif LED detectors highly sensitive compared to detectors based on xenon lamps.

FEATUREs
  • High Sensitivity: 10 times to 100 times greater than conventional fluorescence and up to 100,000 times more sensitive than UV detection
  • Modularity: Adaptable to CE and HPLC systems including Fast-LC, Capillary LC and nano LC
  • Selectivity: Low noise and interference molecules will not be detected, only labelled molecules
  • High efficiency Native fluorescence: 275 nm LED-based detector surpasses existing performances at a reduced cost.

Wavelength - Application

Zetalif LED detectors offer a wide range of LEDs covering various applications fields:

  • 275 nm for amino acids and proteins
  • 365 nm for mono and polysaccharides
  • 450 nm for amino acids, mono and polysaccharides
  • 480 nm for amino acids, DNA/RNA, peptides, oligonucleotides
  • 530 nm for immunoglobulins, IgG, oligonucleotides
  • 640 nm for oligonucleotides.
Download ZETALIF LED Datasheet

ZETALIF Laser

Zetalif Laser induced fluorescence (LIF) detectors are ultra sensitive solutions for all separation techniques such as Liquid Chromatography (capillary LC, microLC, nanoLC, UPLC) and Capillary Electrophoresis. The modular design of this detector provides maximum versatility and enables an unrivalled level of sensitivity for fluorescence measurement thanks to a broad range of lasers offering an excitation wavelength from 266nm to 785nm. Analysts can adapt the wavelength of the laser depending on their application, simply by changing the laser and the corresponding filter blocks.

Depending on the exact application, Zetalif Laser detectors achieve the highest sensitivity.

FEATUREs
  • High Sensitivity: 10 times to 100 times greater than conventional fluorescence and up to 100,000 times more sensitive than UV detection
  • Modularity: Adaptable to CE and HPLC systems including Fast-LC, Capillary LC and nano LC
  • Selectivity: Low noise and interference molecules will not be detected, only labelled molecules.

Wavelength - Application

The Zetalif Laser detector is a sensitive solution for HPLC, fast-LC and CE systems and offers a large range of laser wavelengths:

  • 266 nm: native fluorescence detection of proteins
  • 325 nm or 355 nm: mono and polysaccharides labelled with 2AB or 2AA
  • 405 nm or 445 nm: analysis of amino acids labelled with NDA
  • 488 nm: DNA/RNA, immunoglobins, IgG, amino acids, oligonucleotides polysaccharides…
  • 515 nm or 532 nm: immunoglobins, IgG tagged with 5-Tamra-SE
  • 594 nm: analysis of oligonucleotides labelled with Cy3
  • 642 nm: oligonucleotides labelled with Cy5.

For other wavelengths, please contact us.

Download ZETALIF Laser Datasheet

Interface with CE, LC, MS

Interface with CE systems
  • With Agilent Technologies 7100 CE (www.agilent.com)
  • With other CE: PrinCE Next, WynSep Wyn-CE

For other CE, please contact us

Interface with LC systems
  • Compatible with all LC systems (micro, fast-LC)
  • Optimal sensitivity is achieved at flow rates <0.5 mL/min
Interface with MS systems
  • Online CE-LIF-MS
  • Online LC-LIF-MS
  • Detection cell as close as possible to MS system.
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Main office:

478 rue de la Découverte
31670 LABEGE – France
Tel. +33 (0)5 31 61 50 90

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