MULTI CHANNEL CE

The Multi Channel CE is a versatile four-channel Capillary Electrophoresis Instrument equipped with LEDIF detection.

Overview

The platform includes several innovative features to offer together a great flexibility in method design, a rigorous control of the applied physical parameters, and precision in the measurements.

Key features of the platform:

Four channels run in parallel; effective capillary length of 13 and 24 cm
LED induced fluorescence detection, with the well-known sensitivity of Picometrics Zetalif^TM detectors
Voltage from -30 to +30 kV
Pressure from 20 mbar to 12 bar
Capillary temperature from 20 to 55°C
Samples microplate hold at dew temperature: no evaporation, no condensation, constant sample concentration during waiting
Electric current measurement in each capillary for a better QC
Fluid handling achieved by a robotic pipeting arm; no tubing, no valve, buffer renewal possible for each sample; the fluid handling system works in hidden time compared to voltage and pressure steps
Up to 8 different reagents on board
Full automation.

Capillary cassettes

Capillaries are pre-assembled in ready-to-use cassettes. Two lengths are available:

Short capillaries:

L_tot=22 cm
L_eff=13 cm

Long capillaries:

L_tot=33.3 cm
L_eff=24.1 cm

Installation of the cassettes within the instrument is straightforward, with a self-alignment of the capillary in front of the detection head. There is no need of special tool or alignment procedure.

Sample loading and storage

Samples are loaded in a 96-well microplate, with a minimum of 10 µl.
The temperature of the microplate is regulated at dew point to avoid evaporation while waiting, keeping sample concentration untouched.

Fluid handling

Fluid handling is achieved by a robot which:

Pipet reagents from 15-mL tubes to 8-tube strips. One tip = one reagent
Place/Remove the 8-tube strips to/from the tray under capillary devices
Empty reagents from strips to waste
Fast exchange of strips between successive steps of the analytical process; no drying of capillary edges
Up to 8 reagents possible –> great flexibility in assay design; perfect controls of washes and conditioning
Fresh reagents for each sample –> excellent repeatability
Fluid handling operated in hidden time –> no significant increase of processing time.

Multiple CE modes

Voltage can be applied in one or the other polarity, from to 0 to 30000 V. Electric current is measured in each of the 4 capillaries.
Pression can be applied at inlet, or outlet, or both, from 20 mbar to 12 bars. Two regulators are used for optimal precision, one for low pressures, the other for high pressures.
The instrument contains its own internal pressure source, no need for an external pressure supply.
These features enable any mode of capillary electrophoresis (CZE, CGE, MEKC, CEC, CITP) or Taylor Dispersion Analysis (TDA).

Main specifications

4 capillaries run in parallel	22 or 33 cm long capillary device
LED induced fluorescence detection	Fluorescein excitation and emission wavelength*
Size width x depth x height	90 x 60 x 80 cm
Weight	110 kg
Power supply	110-250 V; 50-60 Hz
Max power consumption	1 kW
Capillary voltage	From -30 to +30 kV
Pressure	20 mbar – 12 bar, at inlet, outlet, or both
Capillary temperature	20 – 55°C
Sample temperature	Dew point
Number of samples	Up to 96
Number of different reagents	Up to 8

*For other wavelengths, please contact us.

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