Amino Acids and Catecholamines Analysis in Microdialysates
High sensitivity separation of primary amino acids and catecholamines
This application allows the simultaneous detection of Amino acids and catecholamines in microdialysates. The method couples capillary HPLC with LIF detection to provide the neuroscientist with one of the most powerful tool for sensitivity in analysis of microdialysates.
Primary amino acids and catecholamines separate in a single run
Limit of detection :
- Glutamate: 4.5 x 10-10 mol/L (4.5 x 10-16 moles)
- Gaba: 1.4 x 10-10 mol/L (1.4 x 10-16 moles)
- Aspartate: 8.5 x 10 -10 mol L (8.5 x 10-16 moles)
(LOD calculated for a S/N ratio of 3)
Run time : 22 minutes
Sample injection of 1 µl
NDA labelling reagent:
NDA is a fluorogenic dye which fluoresces weakly in its native form but has a good fluorescent yield when reacted with CN- and a primary amine. Such derivatives are excitable at 410 nm or 442 nm, using respectively diode or HeCd lasers.
Derivatization procedure: refer to TN002
Instruments | Detector: Picometrics ZETALIF LASER 2000 detector, Laser: 410nm diode Capillary HPLC: Ultimate Dionex LC-Packing system |
Samples | Standards : Amino acids at 10-8 M Microdialysate |
Method | Mobile Phase: A:Sodium citrate buffer 5mM pH=3.2 B : Acetonitrile Flow rate: 5 µL/min, Injection volume: 1 µL, Column: Inertsil ODS3, 3µm 150mm x 300µm GROM |
For Cathecholamines & GABA separation, refer to the Application note AN 025-04B
