
BIABooster Capillary
A proprietary device for injecting, concentrating, and separating DNA
With the BIABooster system DNA concentration as low as 20 pg/ml per fragment can be readily analyzed Thanks to innovative on-line concentration and separation techniques, the BIABooster system enables DNA size profiling with an unrivalled sensitivity. This opens up a new way for characterizing tiny amount of DNA. For the first time, cell-free circulating DNA in plasma, residual DNA in a bioproduct, low input sequencing library… are easy to assay.
The BIABooster is a plug-in for commercial Capillary Electrophoresis Instruments. It works with µLAS technology, in which applying simultaneously hydrodynamic and electric fields in a viscoelastic buffer generates a stable on-line concentration of DNA at the junction between two capillaries of different diameters. After concentration, a progressive change in hydrodynamic and electric fields results in size separation. Separated DNA peaks are detected by laser induced fluorescence.
Up to 1 µl of sample is injected in the device, to be compared to a few nanoliters injected in an usual capillary or microchip electrophoresis. This allows the analysis of very diluted DNA samples, invisible in electrophoresis techniques.
The BIABooster Analytics software has been developed to analyze raw fluorescence data and to translate them into fully informative concentration distribution according to DNA size.
This intuitive and user-friendly software contains key features for automating or streamlining QC of the experiments, data analysis, sample comparisons, export of graphs and data, and reporting.
A proprietary device for injecting, concentrating, and separating DNA
Contains all reagents & plastic wares to analyze 50 samples.
Holds the BIABooster Capillary in the CE machine Translates raw fluorescence traces into a quantified mass distribution according to sizeBIABooster Cassette
Agilent 7100 CE + Zetalif Detector
BIABooster Analytics Software
Getting a DNA profile of plasmatic free DNA is almost impossible using existing electrophoresis systems.
But it is an easy thing with the BIABooster!
Circulating Cell-free DNA (ccfDNA) is now recognized as a potential biomarker enabling personalized medicine for a wide range of pathologies. The BIABooster can be used both to QC DNA samples before PCR or sequencing, and to study ccfDNA size profile according to clinical situations.
With the BIABooster system DNA concentration as low as 20 pg/ml per fragment can be readily analyzed Thanks to innovative on-line concentration and separation techniques, the BIABooster system enables DNA size profiling with an unrivalled sensitivity. This opens up a new way for characterizing tiny amount of DNA. For the first time, cell-free circulating DNA in plasma, residual DNA in a bioproduct, low input sequencing library… are easy to assay.
Adelis developed a LIF Driver that allows for a full integration of all aspects of the fluorescence detector into the powerful OpenLAB CDS ChemStation of Agilent Technologies software.
Zetalif Laser induced fluorescence (LIF) detectors are ultra sensitive solutions for all separation techniques such as Liquid Chromatography (capillary LC, microLC, nanoLC, UPLC) and Capillary Electrophoresis. The modular design of this detector provides maximum versatility and enables an unrivalled level of sensitivity for fluorescence measurement thanks to a broad range of lasers offering an excitation wavelength from 266nm to 785nm. Analysts can adapt the wavelength of the laser depending on their application, simply by changing the laser and the corresponding filter blocks.
The Zetalif LED induced fluorescence (LEDIF) detectors are sensitive solutions for all separation techniques such as Liquid Chromatography (capillary LC, microLC, nanoLC, UPLC) and Capillary Electrophoresis. A large range of LED wavelengths is available: 275, 365, 450, 480, 530 and 640 nm. Adelis developed this LED-based detector to propose the analysts almost equal sensitivity as laser technology with a significantly reduced cost.