Monday, September 16, come and meet Frédéric Ginot for a lecture on the practical applications of DNA analysis by electro-hydrodynamic actuation, focusing on examples where no prior purification of DNA is required.
This innovative feature has been leveraged in a commercial capillary electrophoresis (CE) instrument, achieving DNA separation with remarkable sensitivity—down to 1 femtogram per microliter. The method features a sizing reproducibility and accuracy better than 3% within the 0.1 to 1.5 kb range.
These performances enable concentration and size distribution analysis of circulating DNA in plasma without the need for purification, a procedure that is now being utilized in large-scale clinical trials and can be applied to residual DNA in a bioproduct.
Furthermore, unlike standard capillary gel electrophoresis (CGE), dual hydrodynamic and electrokinetic actuation allows the analysis of large DNA fragments, exceeding 100 kbp without any compromise in accuracy and reproducibility. This capability is crucial for the rapid assessment of genomic DNA extraction quality, particularly when preparing for long-read sequencing.
