Detected molecules : Monoclonal antibody (IgG)
Separation device : CE/LEDIF
Excitation wavelength : 480/530
Dye : FQ/TAMRA
Monoclonal antibodies have become a fast growing class of biopharmaceutical products.
To support analytical characterization in process development and quality control of therapeutic antibodies, capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) has been recognized as an important tool in place of SDS-Page because of the ease of use and the ability to automate.
In this application note, CE derivatization technique for LED Induced Fluorescence detection have been tested to increase detection sensitivity of proteins with the use of 5-carboxytetramethylrhodamine succinimidyl ester (5-TAMRA.SE) and 3-(2-furoyl)-quinoline-2 carboxaldehyde (FQ) as derivatizing agents.