Detected molecules : Monoclonal antibody (IgG)
Separation device : CE/LEDIF
Excitation wavelength : 275
Dye : Native Fluorescence
Pharmaceutical antibodies are a class of therapeutics that plays an important role in controlling many types of diseases such as cancers, allergy, inflammation, infectious or autoimmune diseases. Monoclonal antibodies have become a major class of biopharmaceutical products. There is an increasing demand in the pharmaceutical industry for tools applicable to large scale production and for product characterization and control of the manufacturing process. To support analytical characterization, process development and quality control of therapeutic antibodies in the biopharmaceutical industry, capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) has been recognized as a significant alternative tool of SDS Page because of its ease of use and ability to automate.
This application note describes the analytical methodology for quality control analyses of IgG and their impurities using a 275nm LEDIF detector.