Detected molecules : Monoclonal antibody (IgG)
Separation device : CE/LIF
Excitation wavelength : 266/488/532
Dye : Native fluorescence/FITC/TAMRA
Antibody pharmaceutical is a category of therapeutics that plays an important role in controlling many types of diseases such as cancer, allergy, inflammation, infectious or autoimmune diseases. Monoclonal antibodies have become a fast growing class of biopharmaceutical products. There is an increased demand in the pharmaceutical industry for tools applicable to large scale production and there is an increasing need for product characterization and control of the manufacturing process. In the biopharmaceutical industry, to support analytical characterization, process development, and quality control of therapeutic antibodies, capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) has been recognized as an important alternative tool of SDS-Page because of the ease of use and the ability to automate.
In this application note, CE derivatization technique for Laser Induced Fluorescence detection has been tested to increase detection sensitivity of proteins. We present 2 different LIF detections : Fluorescence derivatization and native fluorescence.