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DNA 1K Kit

01 Overview

The DNA 1K kit is designed to determine the size profile of double stranded DNA samples in the 0.1 to 1.5 kb range with the BIABooster system. The kit is designed for 50 samples, excluding standards. It is also well suited for circulating cell-free DNA. The unrivaled sensitivity of the method makes possible to fully characterize your cfDNA samples using only a tiny amount of it.

Typical cfDNA size profile of a healthy donor

cfDNA concentration

02 Specifications

Analytical SpecificationsDNA 1K Kit
Sizing Range0.1-1.5 kb
Limit of Detection (LOD) (1)10 pg/mL at 1kb
50 pg/mL at 200 bp
Sizing Accuracy± 3%
Sizing Reproducibility< 3% CV
Quantitative Range for DNA Fragments40 pg/mL to 10 ng/mL
Quantitative Range for Biological Samples5 – 1000 ng/mL for cfDNA
Quantification Accuracy± 20%
Quantification Precision< 20% CV
Sample Volume10 µL (1 µL injected)
Salt Concentration (2)0-15 mM for LoSalt method
0-130 mM for HiSalt method
Turn Around Time (LoSalt method)37 minutes
Kit Stability 2 years

 

1 Limit of detection : concentration for that signal to noise ratio (SNR) equals 3. Signal : height of the fragment peak. Noise : peak-to-peak noise measured on the baseline.
2 For NaCl in TE

03 Kit Content

The DNA 1K kit includes:

  • Running buffer DNA 1K, 40 mL
  • Capillary conditioning solution, 10 mL
  • Fluorescent dye, 10 tubes of 25 µL
  • DNA ladder, 3 tubes of 55 µL
  • Filters and syringes, 10 pcs
  • Glass inserts, 65 pcs

Standard ladder used in the DNA 1K kit. Total ladder concentration is 32 ng/mL.

dna 1k ladder
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