Detected molecules : Carbohydrate
Separation device : CE/LIF/MS
Excitation wavelength : 488
Dye : APTS
Glycan characterization of therapeutic proteins is of utmost importance due to the role of carbohydrates in protein stability, half-life, efficacy and mechanism of action. This application note presents the on-line CE-LIF-MS technology for the direct identification of N-linked glycan profiling of rMAbs. CE-MS technology with on-line LIF detection has been developed to provide accurate mass information on major and minor peaks observed in the routine CE-LIF assay.