BIABooster Kits for DNA Sizing

01 Overview

Sensitivity and precision

The BIABooster kits for DNA sizing offer an unrivaled sensitivity for DNA size analysis. This outstanding sensitivity is based on µLAS technology, which enables simple and efficient on-line concentration. Precise quantification of DNA size and concentration are obtained using carefully manufactured DNA standards.

 

Ease of use

Samples are loaded in the system as they are; no loading buffer needs to be added to the samples.

The kits contain all the reagents and disposables required for the analysis of 50 samples.

The shelf life is 1 year minimum.

02 Specifications

Analytical SpecificationsDNA 1K KitDNA 10K Kit
Sizing Range0.1-1.5 kb1-10 kb
Limit of Detection (LOD) (1)10 pg/mL at 1kb
50 pg/mL at 200 bp
10 pg/mL at 1kb
50 pg/mL at 200 bp
Sizing Accuracy± 3%± 3% from 1 to 6 kb
± 5% from 6 to 10 kb
Sizing Reproducibility< 3% CV< 3% CV
Quantitative Range for DNA Fragments40 pg/mL to 10 ng/mL40 pg/mL to 10 ng/mL
Quantitative Range for Biological Samples5 – 1000 ng/mL for cfDNA0.5 – 400 ng/mL for DNA smear
Quantification Accuracy± 20%± 20%
Quantification Precision< 20% CV< 20% CV
Sample Volume10 µL (1 µL injected)10 µL (1 µL injected)
Salt Concentration (2)0-15 mM for LoSalt method
0-130 mM for HiSalt method
0-15 mM
Turn Around Time (LoSalt method)37 minutes70 minutes
Kit Stability 2 years> 6 months
Aging tests in progress

 

1 Limit of detection : concentration for that signal to noise ratio (SNR) equals 3. Signal : height of the fragment peak. Noise : peak-to-peak noise measured on the baseline.
2 For NaCl in TE

Ask for a quotation

03 Related Products