Detected molecules : Amino acids & Catecholamines
Separation device : Fast LC/Capil.LC/CE
Excitation wavelength : 410
Dye : NDA
This application note presents the comparison between UPLC/LIF, capillary LC/LIF and CE/LIF analysis of 20 primary amino acids and catecholamines labeled with NDA in microdialysates. It permits to do the best compromise for the neuroscientist needs. The three methods were optimisated to show the main amino acids and catecholamines in neurobiological samples, but they are adjustable, depending on what amino acids the neurochemist need for his study.
We used NDA (naphthalene-2,3-dicarboxyaldehyde) because it perfectly allows derivatizing primary amines containing molecules at concentrations as low as 100 picomolar.