Detected molecules : Amino acids & Catecholamines
Separation device : CE
Excitation wavelength : 410 or 442
Dye : NDA
This application note presents the separation of 17 amino acids (all the common amino acids, except Pro and Lys) labeled with naphthalene-2,3-dicarboxyaldehyde (NDA) using a Waters® ACQUITY UPLC™ System (Waters Corp., Milford MA) with a 1 or 2 mm micro-column and detection with a ZETALIF Laser Induced fluorescence detector. The linear velocity in the capillary is optimized and subnanomolar LODs are reached.